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cell signaling technology dm1a  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signaling technology dm1a
    Cell Signaling Technology Dm1a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+tubulin+dm1a+cell+signaling+technology/alpha-Tubulin+Mouse+mAb/pmc12037915__41598_2025_95053_MOESM2_ESM-1-146-146
    Average 97 stars, based on 1550 article reviews
    cell signaling technology dm1a - by Bioz Stars, 2026-09
    97/100 stars

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    Western Blot:

    Article Title: Tumour-intrinsic endomembrane trafficking by ARF6 shapes an immunosuppressive microenvironment that drives melanomagenesis and response to checkpoint blockade therapy
    Article Snippet: Key resource table Antibodies Target Antigen Fluorophore Clone Vendor Catalog# Working Dilution Antibodies (Flow Cytometry-Cell surface staining) B220 FITC RA3-6B2 BioLegend 103206 1:400 CD3 Percp-cy5.5 17A2 BioLegend 100218 1:200 CD3 PE 17A2 BioLegend 100205 1:400 CD4 AF700 GK1.5 BioLegend 100430 1:400 CD45 BV711 30-F11 BioLegend 103147 1:1000 CD8a PE/Cy7 53-6.7 BioLegend 100722 1:200 CD8a APC 53-6.7 BioLegend 100712 1:200 CD11b APC/Cy7 M1/70 BioLegend 101226 1:200 CD11c PE N418 BioLegend 117308 1:200 CD16/32 - S17011E BioLegend 156604 1:200 F4/80 APC BM8 BioLegend 123130 1:400 IFN!R1(CD119) PE 2E2 ThermoFisher A16396 1:200 IFN!R1(CD119) PE GIR-94 BioLegend 308704 1:200 IFN!R1(CD119) PE GIR-208 BioLegend 308606 1:200 Ly6C BV421 1A8 BioLegend 127628 1:200 Ly6G BV605 HK1.4 BioLegend 128035 1:200 MHCII BV510 M5/114.15.2 BioLegend 107636 1:1000 NK1.1 Percp PK136 BioLegend 108725 1:200 PD-1 PE/Cy7 29F.1A12 BioLegend 135216 1:200 PD-L1(CD274) PE 10F.9G2 BioLegend 124308 1:200 H-2Kd/H-2Dd PE 34-1-2S BioLegend 114708 1:100 IgG2a,k isotype Ctrl PE MOPC-173 BioLegend 400212 1:100 Human CD119 PE GIR-208 BioLegend 308606 1:100 Antibodies (Flow Cytometry-Intracellular staining) Foxp3 FITC FJK-16s BioLegend 11-5773-82 1:200 Foxp3 eFluor506 FJK-16s ThermoFisher 69-5773-82 1:200 GzmB PE NGZB ThermoFisher 12-8898-82 1:200 GzmB APC NGZB ThermoFisher 17-8898-82 1:200 IFN! .. FITC XMG1.2 BioLegend 505806 1:100 IFN!R1(CD119) PE 2E2 Abcam ab95673 1:100 PD-L1(CD274) PE 10F.9G2 BioLegend 124308 1:200 Antibodies (Western Blot-Primary antibodies) ARF6 - D12G6 Cell Signaling Technology 5740s 1:1000 a-tubulin - DM1A Cell Signaling Technology 3873s 1:1000 ARF1 - - Invitrogen PA1-127 1:1000 GAPDH - D16H11 Cell Signaling Technology 5174 1:10000 IFN!R1 - EPR24127-89 Abcam ab280353 1:1000 IFN!R1 - D-3 Santa Cruz Biotechnology SC-28363 1:1000 IDO - D5J4E Cell Signaling Technology 86630S 1:1000 IDO - D8W5E Cell Signaling Technology 5185S 1:1000 CD80 - E6J6N Cell Signaling Technology 54521S 1:1000 Chemical Compounds Oligonucleotides JAK1 - 6G4 Cell Signaling Technology 3344S 1:1000 p-JAK1(Tyr1034/1035) - D7N4Z Cell Signaling Technology 74129S 1:1000 Lamp1 - D2D11 Cell Signaling Technology 9091S 1:1000 PD-L1 - D4H1Z Cell Signaling Technology 60475S 1:1000 PD-L1 - E1L3N Cell Signaling Technology 13684S 1:1000 STAT1 - D1K9Y Cell Signaling Technology 14994S 1:1000 p-STAT1(Tyr701) - D4A7 Cell Signaling Technology 7649S 1:1000 LSECtin - EPR13724 Abcam ab181196 1:1000 Galectin-3/LGALS3 - - Cell Signaling Technology 12733S 1:1000 Galectin-3/LGALS3 - D4I2R Cell Signaling Technology 87985S 1:1000 CYTH1 - 2E11 ThermoFisher MA1-060 1:1000 DYKDDDDK Tag - D6W5B Cell Signaling Technology 14793S 1:1000 Antibodies (Western Blot-Secondary antibodies) IgG - Jackson ImmunoResearch 715-035-152 1:5000 IgG - Jackson ImmunoResearch 711-035-152 1:5000 (1:10000 only for GAPDH) Chemical compound Vendor Catalog# Recombinant Murine IFN! .. PeproTech 315-05 Recombinant Human IFN!

    Recombinant:

    Article Title: Tumour-intrinsic endomembrane trafficking by ARF6 shapes an immunosuppressive microenvironment that drives melanomagenesis and response to checkpoint blockade therapy
    Article Snippet: Key resource table Antibodies Target Antigen Fluorophore Clone Vendor Catalog# Working Dilution Antibodies (Flow Cytometry-Cell surface staining) B220 FITC RA3-6B2 BioLegend 103206 1:400 CD3 Percp-cy5.5 17A2 BioLegend 100218 1:200 CD3 PE 17A2 BioLegend 100205 1:400 CD4 AF700 GK1.5 BioLegend 100430 1:400 CD45 BV711 30-F11 BioLegend 103147 1:1000 CD8a PE/Cy7 53-6.7 BioLegend 100722 1:200 CD8a APC 53-6.7 BioLegend 100712 1:200 CD11b APC/Cy7 M1/70 BioLegend 101226 1:200 CD11c PE N418 BioLegend 117308 1:200 CD16/32 - S17011E BioLegend 156604 1:200 F4/80 APC BM8 BioLegend 123130 1:400 IFN!R1(CD119) PE 2E2 ThermoFisher A16396 1:200 IFN!R1(CD119) PE GIR-94 BioLegend 308704 1:200 IFN!R1(CD119) PE GIR-208 BioLegend 308606 1:200 Ly6C BV421 1A8 BioLegend 127628 1:200 Ly6G BV605 HK1.4 BioLegend 128035 1:200 MHCII BV510 M5/114.15.2 BioLegend 107636 1:1000 NK1.1 Percp PK136 BioLegend 108725 1:200 PD-1 PE/Cy7 29F.1A12 BioLegend 135216 1:200 PD-L1(CD274) PE 10F.9G2 BioLegend 124308 1:200 H-2Kd/H-2Dd PE 34-1-2S BioLegend 114708 1:100 IgG2a,k isotype Ctrl PE MOPC-173 BioLegend 400212 1:100 Human CD119 PE GIR-208 BioLegend 308606 1:100 Antibodies (Flow Cytometry-Intracellular staining) Foxp3 FITC FJK-16s BioLegend 11-5773-82 1:200 Foxp3 eFluor506 FJK-16s ThermoFisher 69-5773-82 1:200 GzmB PE NGZB ThermoFisher 12-8898-82 1:200 GzmB APC NGZB ThermoFisher 17-8898-82 1:200 IFN! .. FITC XMG1.2 BioLegend 505806 1:100 IFN!R1(CD119) PE 2E2 Abcam ab95673 1:100 PD-L1(CD274) PE 10F.9G2 BioLegend 124308 1:200 Antibodies (Western Blot-Primary antibodies) ARF6 - D12G6 Cell Signaling Technology 5740s 1:1000 a-tubulin - DM1A Cell Signaling Technology 3873s 1:1000 ARF1 - - Invitrogen PA1-127 1:1000 GAPDH - D16H11 Cell Signaling Technology 5174 1:10000 IFN!R1 - EPR24127-89 Abcam ab280353 1:1000 IFN!R1 - D-3 Santa Cruz Biotechnology SC-28363 1:1000 IDO - D5J4E Cell Signaling Technology 86630S 1:1000 IDO - D8W5E Cell Signaling Technology 5185S 1:1000 CD80 - E6J6N Cell Signaling Technology 54521S 1:1000 Chemical Compounds Oligonucleotides JAK1 - 6G4 Cell Signaling Technology 3344S 1:1000 p-JAK1(Tyr1034/1035) - D7N4Z Cell Signaling Technology 74129S 1:1000 Lamp1 - D2D11 Cell Signaling Technology 9091S 1:1000 PD-L1 - D4H1Z Cell Signaling Technology 60475S 1:1000 PD-L1 - E1L3N Cell Signaling Technology 13684S 1:1000 STAT1 - D1K9Y Cell Signaling Technology 14994S 1:1000 p-STAT1(Tyr701) - D4A7 Cell Signaling Technology 7649S 1:1000 LSECtin - EPR13724 Abcam ab181196 1:1000 Galectin-3/LGALS3 - - Cell Signaling Technology 12733S 1:1000 Galectin-3/LGALS3 - D4I2R Cell Signaling Technology 87985S 1:1000 CYTH1 - 2E11 ThermoFisher MA1-060 1:1000 DYKDDDDK Tag - D6W5B Cell Signaling Technology 14793S 1:1000 Antibodies (Western Blot-Secondary antibodies) IgG - Jackson ImmunoResearch 715-035-152 1:5000 IgG - Jackson ImmunoResearch 711-035-152 1:5000 (1:10000 only for GAPDH) Chemical compound Vendor Catalog# Recombinant Murine IFN! .. PeproTech 315-05 Recombinant Human IFN!



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    Figure 1. PROTAC molecules bind with CD36 (A) Structure of trivalent biotin probe, BRD-VHL-biotin. The BRD4 binding motif, VHL E3 ligase binding motif, D-(+)-biotin motif, and linker are colored red, blue, purple, and black, respectively. Compound structure is represented by tethered triangles and a cycle. (B) Workflow for detecting membrane targets of BRD4-VHL-biotin. (C) Structures of trivalent PROTACs SIM1 and SIM1-Me. BRD4 binding motifs in SIM1 or SIM1-Me, VHL E3 ligase binding motifs, and linkers are colored as red, blue, and black, respectively. Compound structure is represented by tethered triangles. (D) Densitometric analyses (left) and a representative immunoblotting assay (right) showing the relative expression of CD36 in membrane and cytosolic fractions from LNCaP cells. CD36 expression in membrane and cytosolic fractions from LNCaP cells (right) was examined by immunoblotting assays and normalized to <t>a-tubulin</t> for densitometric analyses (left). Data are mean ± SEM, n = 3. (E) Biotin pull-down assay showing SIM1-Me is competitive with BRD-VHL-biotin for binding with CD36 in membrane fractions from LNCaP cells under indicated conditions. (F) Representative sensograms from surface plasmon resonance assays showing the binding affinity of SIM1-Me to an hCD36-coated Series S sensor chip CM5 from Cytiva. KD values were calculated by BiacoreTM T200 and GraphPad Software by plotting equilibrium response units against compound concentrations. See also Figure S1.
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    Figure 1. PROTAC molecules bind with CD36 (A) Structure of trivalent biotin probe, BRD-VHL-biotin. The BRD4 binding motif, VHL E3 ligase binding motif, D-(+)-biotin motif, and linker are colored red, blue, purple, and black, respectively. Compound structure is represented by tethered triangles and a cycle. (B) Workflow for detecting membrane targets of BRD4-VHL-biotin. (C) Structures of trivalent PROTACs SIM1 and SIM1-Me. BRD4 binding motifs in SIM1 or SIM1-Me, VHL E3 ligase binding motifs, and linkers are colored as red, blue, and black, respectively. Compound structure is represented by tethered triangles. (D) Densitometric analyses (left) and a representative immunoblotting assay (right) showing the relative expression of CD36 in membrane and cytosolic fractions from LNCaP cells. CD36 expression in membrane and cytosolic fractions from LNCaP cells (right) was examined by immunoblotting assays and normalized to <t>a-tubulin</t> for densitometric analyses (left). Data are mean ± SEM, n = 3. (E) Biotin pull-down assay showing SIM1-Me is competitive with BRD-VHL-biotin for binding with CD36 in membrane fractions from LNCaP cells under indicated conditions. (F) Representative sensograms from surface plasmon resonance assays showing the binding affinity of SIM1-Me to an hCD36-coated Series S sensor chip CM5 from Cytiva. KD values were calculated by BiacoreTM T200 and GraphPad Software by plotting equilibrium response units against compound concentrations. See also Figure S1.
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    Figure 1. PROTAC molecules bind with CD36 (A) Structure of trivalent biotin probe, BRD-VHL-biotin. The BRD4 binding motif, VHL E3 ligase binding motif, D-(+)-biotin motif, and linker are colored red, blue, purple, and black, respectively. Compound structure is represented by tethered triangles and a cycle. (B) Workflow for detecting membrane targets of BRD4-VHL-biotin. (C) Structures of trivalent PROTACs SIM1 and SIM1-Me. BRD4 binding motifs in SIM1 or SIM1-Me, VHL E3 ligase binding motifs, and linkers are colored as red, blue, and black, respectively. Compound structure is represented by tethered triangles. (D) Densitometric analyses (left) and a representative immunoblotting assay (right) showing the relative expression of CD36 in membrane and cytosolic fractions from LNCaP cells. CD36 expression in membrane and cytosolic fractions from LNCaP cells (right) was examined by immunoblotting assays and normalized to <t>a-tubulin</t> for densitometric analyses (left). Data are mean ± SEM, n = 3. (E) Biotin pull-down assay showing SIM1-Me is competitive with BRD-VHL-biotin for binding with CD36 in membrane fractions from LNCaP cells under indicated conditions. (F) Representative sensograms from surface plasmon resonance assays showing the binding affinity of SIM1-Me to an hCD36-coated Series S sensor chip CM5 from Cytiva. KD values were calculated by BiacoreTM T200 and GraphPad Software by plotting equilibrium response units against compound concentrations. See also Figure S1.
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    Figure 1. PROTAC molecules bind with CD36 (A) Structure of trivalent biotin probe, BRD-VHL-biotin. The BRD4 binding motif, VHL E3 ligase binding motif, D-(+)-biotin motif, and linker are colored red, blue, purple, and black, respectively. Compound structure is represented by tethered triangles and a cycle. (B) Workflow for detecting membrane targets of BRD4-VHL-biotin. (C) Structures of trivalent PROTACs SIM1 and SIM1-Me. BRD4 binding motifs in SIM1 or SIM1-Me, VHL E3 ligase binding motifs, and linkers are colored as red, blue, and black, respectively. Compound structure is represented by tethered triangles. (D) Densitometric analyses (left) and a representative immunoblotting assay (right) showing the relative expression of CD36 in membrane and cytosolic fractions from LNCaP cells. CD36 expression in membrane and cytosolic fractions from LNCaP cells (right) was examined by immunoblotting assays and normalized to a-tubulin for densitometric analyses (left). Data are mean ± SEM, n = 3. (E) Biotin pull-down assay showing SIM1-Me is competitive with BRD-VHL-biotin for binding with CD36 in membrane fractions from LNCaP cells under indicated conditions. (F) Representative sensograms from surface plasmon resonance assays showing the binding affinity of SIM1-Me to an hCD36-coated Series S sensor chip CM5 from Cytiva. KD values were calculated by BiacoreTM T200 and GraphPad Software by plotting equilibrium response units against compound concentrations. See also Figure S1.

    Journal: Cell

    Article Title: CD36-mediated endocytosis of proteolysis-targeting chimeras.

    doi: 10.1016/j.cell.2025.03.036

    Figure Lengend Snippet: Figure 1. PROTAC molecules bind with CD36 (A) Structure of trivalent biotin probe, BRD-VHL-biotin. The BRD4 binding motif, VHL E3 ligase binding motif, D-(+)-biotin motif, and linker are colored red, blue, purple, and black, respectively. Compound structure is represented by tethered triangles and a cycle. (B) Workflow for detecting membrane targets of BRD4-VHL-biotin. (C) Structures of trivalent PROTACs SIM1 and SIM1-Me. BRD4 binding motifs in SIM1 or SIM1-Me, VHL E3 ligase binding motifs, and linkers are colored as red, blue, and black, respectively. Compound structure is represented by tethered triangles. (D) Densitometric analyses (left) and a representative immunoblotting assay (right) showing the relative expression of CD36 in membrane and cytosolic fractions from LNCaP cells. CD36 expression in membrane and cytosolic fractions from LNCaP cells (right) was examined by immunoblotting assays and normalized to a-tubulin for densitometric analyses (left). Data are mean ± SEM, n = 3. (E) Biotin pull-down assay showing SIM1-Me is competitive with BRD-VHL-biotin for binding with CD36 in membrane fractions from LNCaP cells under indicated conditions. (F) Representative sensograms from surface plasmon resonance assays showing the binding affinity of SIM1-Me to an hCD36-coated Series S sensor chip CM5 from Cytiva. KD values were calculated by BiacoreTM T200 and GraphPad Software by plotting equilibrium response units against compound concentrations. See also Figure S1.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-a-Tubulin (DM1A) Cell Signaling Technology Cat# 3873; RRID: AB_1904178 Mouse monoclonal anti-b-actin (AC-15) Sigma-Aldrich Cat# A5441; RRID: AB_476744 Mouse Monoclonal Anti- AR (441) Santa Cruz Biotechnology Cat# sc-7305; RRID: AB_626671 Mouse Monoclonal Anti- BRD4 (A-7) Santa Cruz Biotechnology Cat# sc-518021; RRID: AB_2861151 Mouse Monoclonal Anti-EEA1 BD Biosciences Cat# 610457; RRID: AB_397830 Rabbit Monoclonal Anti-AR (ER179(2)) Abcam Cat# ab108341; RRID: AB_10865716 Rabbit Monoclonal Anti-BRD4 (EPR5150(2)) Abcam Cat# ab128874; RRID: AB_11145462 Rabbit onoclonal anti-CD36 (EPR6573) Abcam Cat# ab133625; RRID: AB_2716564 Rabbit monoclonal anti-CD36 (EPR22509-40) Abcam Cat# ab252922; RRID: AB_2922821 Rabbit polyclonal anti- GAPDH (D16H11) Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Rabbit monoclonal anti-Rab5A Cell Signaling Technology Cat# 2143; RRID: AB_823625 Biological samples Prostate cancer tissue microarray TissueArray Cat# PR1921c Chemicals, peptides, and recombinant proteins BDP FL NHS ester Lumiprobe Cat# 61420 Birinapant (CAS # 1260251-31-7) MedChemExpress Cat# HY-16591 Cell proliferation reagent WST-1 Roche Cat# 05015944001 Clarity max western ECL substrate kit BIO-RAD Cat# 1705062 D-(+)-Biotin (CAS # 58-85-5) Thermo Fisher Scientific Cat# BP232-1 D-(+)-Biotin NHS (CAS # 35013-72-0) Combi-Blocks Cat# QA-1959 Doxorubicin (CAS # 23214-92-8) Selleckchem Cat# E2516 Dynabeads MyOne streptavidin T1 Thermo Fisher Scientific Cat# 65601 Human recombinant CD36 protein, ECD, His Tag (HPLC verified) Sino Biological Cat# 10752-H08H Human recombinant CD36 protein, ECD, His Tag (HPLC verified) ACRO Biosystems Cat# CD6-H5221 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Magic dynabeads MyOne streptavidin T1 Thermo Fisher Scientific Cat# 65601 Mem-PER plus membrane protein extraction kit Thermo Fisher Scientific Cat# 89842 Navitoclax (CAS # 923564-51-6) APExBIO Cat# A3007 NE-PER nuclear and cytoplasmic extraction reagents Thermo Fisher Scientific Cat# 78833 Paclitaxel (CAS # 33069-62-4) Selleckchem Cat# S1150 Polybrene Sigma-Aldrich Cat# TR1003 ProlongGold Life Technologies Cat# P36930 Prostate adenocarcinoma tissue microarray TissueArray Cat# PR1921c Protease inhibitor cocktail Roche Cat# 04693132001 Rapalink-1 (CAS # 1887095-82-0) MedChemExpress Cat# HY-111373 Rapamycin (CAS # 53123-88-9) MedChemExpress Cat# HY-10219 Thiazolyl blue tetrazolium bromide Sigma-Aldrich Cat# M5655 (Continued on next page) e1 Cell 188, 1–19.e1–e6, June 12, 2025

    Techniques: Binding Assay, Membrane, Western Blot, Expressing, Pull Down Assay, SPR Assay, Software